ID :
ath-MIR156a
specie :
Arabidopsis thaliana
gene cercato :
SPL3
descrizione :
Arabidopsis thaliana miR156a stem-loop
Commenti :
MIR156a is found on chromosome 2 in Arabidopsis thaliana
.
nome del gene :
SPL3
UTR :
TAAAGACGGTGAAACGTGTGAGATCCCGGTTTGAAGGTTAATGAAACAGG
CTTTGCTTACTCTCTTCTGTCAGTCTCTTTTAGCTCCTTGTAATCCTCTG T
GTCTCTGTCTGTTTCTCCATATTACCTGTAATCAAAGCTATCTGCTAAAC C
TACGACATGGTTAAATAAATGCATTGAGACTTAGTAAATGCTTGAAGAAA T
TATGTGTAATAACTCTTTAAACTTGTCAAATGTTATTTCAAGCC
Target Interaction network :
http://mirtarbase.mbc.nctu.edu.tw/tm...4629075798.png
target dell'autore:
GCTTTGCTTACTCTCTTCTGTCAGTC
con posizione
50
Articolo :
miRNAs are a class of versatile small RNAs that control
gene expression post-transcriptionally, governing many
facets of plant cell functions. They interact with
their target mRNA at a site of sequence complementarity
and modulate their expression levels. Here, we provide
evidence, based on transient assays and stable transgenic
lines, that the 3' UTR of the Arabidopsis SBP box gene
SPL3 contains a functional miRNA-responsive element
(MRE) that is complementary to miR156 and miRNA157.
Seedlings of transgenic lines constitutively over-expressing
an SPL3 transgene either carrying an unaltered or a
disrupted MRE accumulate considerable levels of SPL3
transcripts. However, while the unaltered MRE UTR does
not allow the expression of detectable levels of SPL3
protein, the altered MRE does. Translational inhibition
thus provides an important mechanism for miRNA-mediated
post-transcriptional repression of SPL3. As a consequence
of precocious translation of the constitutively expressed
SPL3 transgene, due to the absence of a functional
MRE, plants exhibit very early flowering in addition
to frequent morphological changes.
Interazione
miRNA 3' caCGAGUGAGAGAAGACAGu 5'
|||:|||||||||||||
Target 5' uuGCUUACUCUCUUCUGUCa 3'
metodi usati :
GFP reporter assay