Ciao Luciano79,
Il seguente è un esempio trovato tempo fa sulla rete, è funzionante.
Inserisci dei controlli sulla Form ... e poi fa click sulla medesima.
codice:
Private Const REG_OR = 2
Private Const REG_MIN = 4
Private Const HWND_TOPMOST = -1
Private Const HWND_NOTOPMOST = -2
Private Const SWP_NOMOVE = &H2
Private Const SWP_NOSIZE = &H1
Private Const TOPMOST_FLAGS = SWP_NOMOVE Or SWP_NOSIZE
Private Declare Function CreateRectRgn Lib "gdi32" (ByVal X1 As Long, ByVal Y1 As Long, ByVal X2 As Long, ByVal Y2 As Long) As Long
Private Declare Function CombineRgn Lib "gdi32" (ByVal hDestRgn As Long, ByVal hSrcRgn1 As Long, ByVal hSrcRgn2 As Long, ByVal nCombineMode As Long) As Long
Private Declare Function SetWindowRgn Lib "user32" (ByVal hwnd As Long, ByVal hRgn As Long, ByVal bRedraw As Boolean) As Long
Private Declare Function DeleteObject Lib "gdi32" (ByVal hObject As Long) As Long
Private Declare Function SetWindowPos Lib "user32" (ByVal hwnd As Long, ByVal hWndInsertAfter As Long, ByVal x As Long, Y, ByVal cx As Long, ByVal cy As Long, ByVal wFlags As Long) As Long

Dim GlassForm As Long
'-----------------------------------------------------------------------------
Private Sub Form_Click()
TraspGlass 1
' TraspGlass 0 x tornare allo stato precedente non-glass
' TraspGlass 1 ' ...Glass PARZIALE (Resta la caption del form)
' TraspGlass 2 ' ...Glass TOTALE (restano solo i controlli senza form)
End Sub
'-----------------------------------------------------------------------------
Private Sub Form_Initialize()

    Me.Height = 3600
    Me.Width = 4800
    
    Me.ScaleMode = vbPixels     ' Non modificare!!!
    Me.Caption = "Un click con il tasto del mouse su questo form..."

End Sub
'-----------------------------------------------------------------------------
Private Sub TraspGlass(GlassFX As Integer)

    Dim w As Single, h As Single, Contorno As Single, topEye As Single
    Dim i As Long, k As Long, n As Long, q As Long
    Dim PosTop As Double, PosLeft As Double
    
    ' Conversione su assi X/Y | width/height | scalewidth/scaleheight
    w = ScaleX(Width, vbTwips, vbPixels)
    h = ScaleY(Height, vbTwips, vbPixels)
    
    ' Verifica lo stato...
    If GlassFX = 0 Then
        GlassForm = CreateRectRgn(0, 0, w, h)
        SetWindowRgn hwnd, GlassForm, True
        Exit Sub
    End If
    
    GlassForm = CreateRectRgn(0, 0, 0, 0)
    Contorno = (w - ScaleWidth) / 2
    topEye = h - Contorno - ScaleHeight
    
    If GlassFX = 1 Then
        k = CreateRectRgn(0, 0, w, h)
        n = CreateRectRgn(Contorno, topEye, w - Contorno, h - Contorno)
        CombineRgn GlassForm, k, n, REG_MIN
    End If
    
    ' Traspone la nuova regione sul form...
    For i = 0 To Me.Controls.Count - 1
        If Me.Controls(i).Visible = True Then
            PosLeft = ScaleX(Me.Controls(i).Left, Me.ScaleMode, vbPixels) + Contorno
            PosTop = ScaleX(Me.Controls(i).Top, Me.ScaleMode, vbPixels) + topEye
            q = CreateRectRgn(PosLeft, PosTop, _
            PosLeft + ScaleX(Me.Controls(i).Width, Me.ScaleMode, vbPixels), PosTop + ScaleY(Me.Controls(i).Height, Me.ScaleMode, vbPixels))
            CombineRgn GlassForm, q, GlassForm, REG_OR
        End If
    Next
    
    ' Esegue...
    SetWindowRgn hwnd, GlassForm, True

End Sub
'-----------------------------------------------------------------------------
Private Sub Form_Load()
    ' Centra il form
    CentraForm Form1
    ' On Top
    MakeTopMost Me.hwnd
End Sub
'-----------------------------------------------------------------------------
Private Sub Form_QueryUnload(Cancel As Integer, UnloadMode As Integer)
    ' Scarca l'oggetto GlassForm dalla memoria.
    SetWindowRgn hwnd, 0, False
    DeleteObject GlassForm
    ' Scarica il form dalla memoria
    Unload Form1
    Set Form1 = Nothing
End Sub
'-----------------------------------------------------------------------------
Public Sub CentraForm(MyForm As Form)
    ' Centra il form
    MyForm.Top = (Screen.Height - MyForm.Height) / 2
    MyForm.Left = (Screen.Width - MyForm.Width) / 2
End Sub
'-----------------------------------------------------------------------------
Sub MakeNormal(Handle As Long)
    SetWindowPos Handle, HWND_NOTOPMOST, 0, 0, 0, 0, TOPMOST_FLAGS
End Sub
'-----------------------------------------------------------------------------
Sub MakeTopMost(Handle As Long)
    SetWindowPos Handle, HWND_TOPMOST, 0, 0, 0, 0, TOPMOST_FLAGS
End Sub